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Questions related from Adam Klocperk
Hi all, can anyone please tell me if there is a way to see why STRING suggested a particular edge between two of my proteins? When I click the edge, it say, for example, co-expression score 0,7 or...
02 August 2019 4,124 1 View
Good morning all, I was wondering if it's possible to use CIBERSORT (or similar deconvolution algorhitm) on RNAseq data of gut biopsies from humans? We are trying to gauge the presence of immune...
04 January 2019 3,123 1 View
Hello all, the title really says it all. I am analysing T-cells in the context of immune dysregulation and I have developed 5 panels, each with around 10 colors (some, but not all (obviously)...
16 May 2018 972 1 View
Hello everyone! I'm using image cytometry to measure nuclear translocation of RelA in T-cells (CD3+) after stimulation with anti-CD3/28. I know that NFkB phosphorylates within 20 minutes of...
20 July 2017 4,286 2 View
Good afternoon, lovely people of ResearchGate. In my assay on ImageStream, my unstained samples (e.g. when creating compensation matrix) have quite strong positivity in several channels (the...
17 May 2017 4,289 2 View
Greetings. I am staining monocyte derived DCs with a panel that includes Lin (CD3, 16, 19, 20, 56). Weirdly, I get a population of HLA-DR+ CD11c+ cells that are also Lin+. Given that CD11c should...
08 January 2016 7,513 2 View
Greetings! As a part of our research we would like to distinguish between classic monocyte-derived dendritic cells and thymic dendritic cells (both thymus-native and thymus-migratory) in human...
03 June 2015 3,325 3 View
