Adam Klocperk

5 Questions 4 Answers 0 Followers

Questions related from Adam Klocperk

How do I see STRING-db interaction/edge sources?

Hi all, can anyone please tell me if there is a way to see why STRING suggested a particular edge between two of my proteins? When I click the edge, it say, for example, co-expression score 0,7 or...

02 August 2019 4,117 1 View

What tools can I use to analyse high-dimensional data from multi-tube flow cytometry experiments?

Hello all, the title really says it all. I am analysing T-cells in the context of immune dysregulation and I have developed 5 panels, each with around 10 colors (some, but not all (obviously)...

16 May 2018 965 1 View

NFkB translocation dynamic in T-cells

Hello everyone! I'm using image cytometry to measure nuclear translocation of RelA in T-cells (CD3+) after stimulation with anti-CD3/28. I know that NFkB phosphorylates within 20 minutes of...

20 July 2017 4,280 2 View

Avoiding severe autofluorescence in PBMCs prepared for ImageStream analysis?

Good afternoon, lovely people of ResearchGate. In my assay on ImageStream, my unstained samples (e.g. when creating compensation matrix) have quite strong positivity in several channels (the...

17 May 2017 4,284 2 View

Can someone help me with Lin expression by (monocyte derived) DCs?

Greetings. I am staining monocyte derived DCs with a panel that includes Lin (CD3, 16, 19, 20, 56). Weirdly, I get a population of HLA-DR+ CD11c+ cells that are also Lin+. Given that CD11c should...

08 January 2016 7,508 2 View