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Questions related from Abhilash S
As part of my research work, I have to perform TUNEL assay in in vivo. Can anyone suggest me some good kits which are economical, based on their experience?
26 August 2016 7,395 2 View
As part of my research work, I have to perform apoptosis assay with animal tissues. I wish to know, whether Propidium Iodide uptake assay be done in rat heart/ liver tissues.
26 August 2016 1,816 3 View
I am working with cell lines. As part of my work, I wish to isolate the membranes of cells. I wish to know if there is any simple method for cell membrane isolation which can be done without using...
02 March 2016 9,574 1 View
Hello, I am working on cell culture and wish to perform inflammatory assays, especially, COX and LOX. I have found several kits of different companies. Due to financial limitations, I think its...
30 November 2015 4,667 5 View
Hello, I bought Amphotericin B from a company. Their instruction is to dissolve it in DMSO at a concentration of 3 mg/mL. Is it suitable to add 10 microliter of this solution (ie 6 micro gram of...
12 October 2015 839 4 View
I have done simultaneous treatment of two drugs in in vitro studies. Now I have to do pre-treatment studies. In simultaneous treatment I have given both the drugs and check for viability after...
10 August 2015 8,159 6 View
I wish to know if there is any chemical change due to repeated sterilization of buffer solutions.
26 March 2015 4,071 2 View
Can anyone please tell me the effective concentration of trypsin used for trypsinization of H9c2 cells? I had used 1x trypsin from Sigma and got good results. But now I got 10x trypsin and it did...
28 October 2014 4,698 2 View
I have been working with some expression studies and has found some publications with and some others without ladder. Can anyone help me?
02 July 2014 8,346 3 View