15 Questions 7 Answers 0 Followers
Questions related from Abhilash S
As part of my research work, I have to perform TUNEL assay in in vivo. Can anyone suggest me some good kits which are economical, based on their experience?
26 August 2016 7,453 2 View
As part of my research work, I have to perform apoptosis assay with animal tissues. I wish to know, whether Propidium Iodide uptake assay be done in rat heart/ liver tissues.
26 August 2016 1,875 3 View
As part of my research work, I have to perform some gene expression studies with animal tissues. I wish to know whether we can preserve the tissues at ultra low temperatures without RNA later for...
18 August 2016 8,025 3 View
I am working with the effect of certain chemicals in antioxidant system using Cell line models. As part of my work, I have to perform SOD assay. I choose the method of Kakkar et al., (1984), since...
15 July 2016 384 0 View
I am working with cell lines. As part of my work, I wish to isolate the membranes of cells. I wish to know if there is any simple method for cell membrane isolation which can be done without using...
02 March 2016 9,658 1 View
Hello, I am working with some cancer drugs. I wish to do caspase assay as part of my work. I have seen different Research papers dealing with caspase assay, most of them as expression studies....
18 February 2016 586 3 View
Hello, I am working on cell culture and wish to perform inflammatory assays, especially, COX and LOX. I have found several kits of different companies. Due to financial limitations, I think its...
30 November 2015 4,710 5 View
I am working on cell lines. I wish to know if its suitable to keep cell lysates at -20 degree celsius for a period of atleast one week, before performing enzyme analysis with the cell lysates.
23 November 2015 2,124 3 View
Hello, I bought Amphotericin B from a company. Their instruction is to dissolve it in DMSO at a concentration of 3 mg/mL. Is it suitable to add 10 microliter of this solution (ie 6 micro gram of...
12 October 2015 884 4 View
I have done simultaneous treatment of two drugs in in vitro studies. Now I have to do pre-treatment studies. In simultaneous treatment I have given both the drugs and check for viability after...
10 August 2015 8,204 6 View
Can anyone please tell me the best colorimetric methods for the detection of calcium- ATPases and sodium- potassium ATPases?
22 June 2015 6,203 3 View
I wish to know if there is any chemical change due to repeated sterilization of buffer solutions.
26 March 2015 4,109 2 View
Can anyone please tell me the effective concentration of trypsin used for trypsinization of H9c2 cells? I had used 1x trypsin from Sigma and got good results. But now I got 10x trypsin and it did...
28 October 2014 4,772 2 View
Nrf2 expression in cardiomyocytes.
13 August 2014 9,041 1 View
I have been working with some expression studies and has found some publications with and some others without ladder. Can anyone help me?
02 July 2014 8,402 3 View
