12 Questions 6 Answers 0 Followers
Questions related from Abhilash S
As part of my research work, I have to perform TUNEL assay in in vivo. Can anyone suggest me some good kits which are economical, based on their experience?
26 August 2016 7,405 2 View
As part of my research work, I have to perform apoptosis assay with animal tissues. I wish to know, whether Propidium Iodide uptake assay be done in rat heart/ liver tissues.
26 August 2016 1,825 3 View
As part of my research work, I have to perform some gene expression studies with animal tissues. I wish to know whether we can preserve the tissues at ultra low temperatures without RNA later for...
18 August 2016 7,993 3 View
I am working with the effect of certain chemicals in antioxidant system using Cell line models. As part of my work, I have to perform SOD assay. I choose the method of Kakkar et al., (1984), since...
15 July 2016 338 0 View
I am working with cell lines. As part of my work, I wish to isolate the membranes of cells. I wish to know if there is any simple method for cell membrane isolation which can be done without using...
02 March 2016 9,585 1 View
Hello, I am working on cell culture and wish to perform inflammatory assays, especially, COX and LOX. I have found several kits of different companies. Due to financial limitations, I think its...
30 November 2015 4,672 5 View
Hello, I bought Amphotericin B from a company. Their instruction is to dissolve it in DMSO at a concentration of 3 mg/mL. Is it suitable to add 10 microliter of this solution (ie 6 micro gram of...
12 October 2015 843 4 View
I have done simultaneous treatment of two drugs in in vitro studies. Now I have to do pre-treatment studies. In simultaneous treatment I have given both the drugs and check for viability after...
10 August 2015 8,164 6 View
Can anyone please tell me the best colorimetric methods for the detection of calcium- ATPases and sodium- potassium ATPases?
22 June 2015 6,129 3 View
I wish to know if there is any chemical change due to repeated sterilization of buffer solutions.
26 March 2015 4,076 2 View
Can anyone please tell me the effective concentration of trypsin used for trypsinization of H9c2 cells? I had used 1x trypsin from Sigma and got good results. But now I got 10x trypsin and it did...
28 October 2014 4,711 2 View
I have been working with some expression studies and has found some publications with and some others without ladder. Can anyone help me?
02 July 2014 8,355 3 View
