Abdullah Mahmud-Al-Rafat

12 Questions 20 Answers 0 Followers

Questions related from Abdullah Mahmud-Al-Rafat

Any recommended kit for Isoenzyme Analysis?

Hi, I need suggestion for isoenzyme analysis kit. Previously we use 'AuthentiKit (Innovative chemistry, USA)' but it is not available now. Can anyone recommend any other commercially available...

14 June 2019 546 0 View

How to improve rotavirus immunofluorescence with Vero cells?

I am getting very poor amount of virus detection with high titre of virus during immunofluorescence. Asking for your suggestions. I am using Vero cells for rotavirus immunofluorescence though...

10 November 2017 9,886 5 View

Why my Vero cells detach after infection and incubation with overlay media?

I am trying to do plaque assay for rotavirus. Though MA104 is widely used for rotavirus assay however vero cell is also recommended. I am trying with vero cell. But my vero cells detach after few...

12 August 2017 5,171 6 View

Does 20 mM sodium phosphate buffer have any negative effect on mammalian cells?

Usually we use 20 mM sodium phosphate buffer as stock solution and prepare a low concentration saline solution from that but I was wondering, if 20 mM sodium phosphate buffer can directly be used...

26 April 2016 958 1 View

Is 'Plasmocin™ by Invivogen' efficient to remove mycoplasma from virus stock?

It will be helpful if someone share their experience with plasmocin to remove mycoplasma from viruses. According to this article...

31 August 2015 5,131 2 View

Can anyone provide me any guideline or protocol to decontaminate the laboratory area from mycoplasma?

Our samples and some reagents are severely contaminated with mycoplasma. We have to throw them away. Besides this, we have to ensure the laboratory space, equipment are also decontaminated from...

24 May 2015 4,342 4 View

Can anyone suggest the treatment steps if mycoplasma positive is found in biological samples e.g: FBS, Trypsin, Cells, Virus etc.?

What should we do to eradicate mycoplasma presence from our lab and samples?

20 May 2015 6,912 8 View

Is there any problem if FBS is stored at -70° C instead of -20° C temperature?

If FBS is stored at -70° C is there any chance that FBS property could be changed and causes any problems to VERO cells after passage?

16 May 2015 7,195 2 View

What factors can cause false-negative in hemagglutinin (HA) assay

we are getting false negative in virus samples. Please share your ideas. Tried 0.5% chicken RBC, 0.75% guinea pig RBC and 0.4% chicken+guinea pig RBC. Also tried with young chicken blood. every...

01 January 1970 9,277 10 View

How to prevent RBC lysis during separation from whole blood?

After centrifugation my pillete when dissolved in Alsever's solution /PBS the solution looks like blood again. Isn't it indication of RBC lysis? How to prevent it? Following the protocol...

01 January 1970 8,379 9 View

Any report on dengue outbreak by 5th serotype?

Discovery of dengue 5th serotype was published few years back. But I still didn't see any broad discussion abut that serotype. Can anybody confirm about any incident or cases bt Dengue 5th serotype?

01 January 1970 1,642 3 View

RBC isolation for hemagglutinin

Rabbit blood with equal amount alsever's solution centrifuged with 500 g, 20 mins, 4 degree with centrifugal acceleration 6 and deceleration 1. 0.01 M PBS used for washing. After first wash no...

01 January 1970 5,331 0 View