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Questions related from Aakash Mukhopadhyay
I have a CRISPR guide which targets near to the end of Exon 1 of a gene. When I am analysing the sequences my mutants, I can see that there are insertions and deletions in Exon 1, but since the...
27 March 2019 9,283 4 View
I have dynein inhibitors, and want to prove that they inhibit the dyneins of Leishmania (my model organism of interest). I would like to perform an amino acid conservation analysis where I can...
29 November 2017 2,817 2 View
I am trying to measure the amount of inorganic phosphate using the malachite green assay (Carter SG and Karl DW. 1982. J Biochemical Biophysical Methods). They use a sodium molybdate solution...
02 March 2017 5,806 9 View
Can anybody suggest any references where the cell volume of Leishmania promastigotes has been measured?
16 May 2016 2,299 3 View
I have recently come across many papers which have reported the discovery of a new downstream effector protein known as EPAC in the cAMP signaling pathway of mammals. I was wondering whether...
17 April 2016 1,070 0 View
I have a set of buffer solutions containing chelators like EGTA and EDTA. I would still like to know what concentration of calcium is present in the solution (maybe micro or nanomolar range) as my...
15 January 2016 1,906 4 View
My cells do not seem to respond to a concentration as high as 1 mM theophylline.
27 March 2015 8,137 6 View
