Aakash Mukhopadhyay

11 Questions 12 Answers 0 Followers

Questions related from Aakash Mukhopadhyay

Can the effect of a CRISPR mutation on one exon be reflected with a premature stop codon in the adjacent exon?

I have a CRISPR guide which targets near to the end of Exon 1 of a gene. When I am analysing the sequences my mutants, I can see that there are insertions and deletions in Exon 1, but since the...

27 March 2019 9,347 4 View

How to perform amino acid conservation analysis of Dynein heavy chains??

I have dynein inhibitors, and want to prove that they inhibit the dyneins of Leishmania (my model organism of interest). I would like to perform an amino acid conservation analysis where I can...

29 November 2017 2,904 2 View

Does Leishmania have Calcium-calmodulin dependent phosphodiesterase?

I am confused with the nomenclature of the phosphodiesterases (PDEs) in trypanosomes like Leishmania. I am aware that PDEs (A, B1, B2, and C) are present in Leishmania however I am not being able...

10 May 2017 6,690 1 View

How can I isolate Leishmania flagella without any detergent/salt extraction?

I would like to isolate flagella from Leishmania parasites. There are numerous publications that do so, however by procedures that involve detergent and salt extractions to depolymerize the...

21 March 2017 8,606 0 View

How to perform Malachite green assay for studying ATPase activity??

I am trying to measure the amount of inorganic phosphate using the malachite green assay (Carter SG and Karl DW. 1982. J Biochemical Biophysical Methods). They use a sodium molybdate solution...

02 March 2017 5,900 9 View

How to address questions on axonemal dynein heterogeneity?

I have come across publications which hypothesize the presence of different populations of dyneins in the flagellum, which are responsible for regulating the beat/waveform. For eg. Wada et al...

24 October 2016 3,077 4 View

What is the volume of a single Leishmania cell?

Can anybody suggest any references where the cell volume of Leishmania promastigotes has been measured?

16 May 2016 2,368 3 View

Do Trypanosomes (Trypanosoma, Leishmania) possess the EPAC protein as a downstream receptor to cAMP signaling?

I have recently come across many papers which have reported the discovery of a new downstream effector protein known as EPAC in the cAMP signaling pathway of mammals. I was wondering whether...

17 April 2016 1,121 0 View

Can anybody recommend an instrument or kit using with I can measure free calcium concentration at micromolar levels?

I have a set of buffer solutions containing chelators like EGTA and EDTA. I would still like to know what concentration of calcium is present in the solution (maybe micro or nanomolar range) as my...

15 January 2016 1,960 4 View

From where can I purchase the phosphodiesterase inhibitor CpdA?

I came across this trypanosome specific phosphodiesterase inhibitor CpdA in few papers. However I could not find any company which sell the same. Any leads?

01 May 2015 6,543 1 View

Is Theophylline a membrane permeable phosphodiesterase inhibitor?

My cells do not seem to respond to a concentration as high as 1 mM theophylline.

27 March 2015 8,193 6 View