If I don't have DEPC/DEPC treated water, what alternative can I use to make my working pipette, tubes, tips RNase free ? And how?
Hello,
You can wash your bench, pipettes with RNase erase solution (commercial). Make an autoclave of you milliQ water, and use the filtered tips and tubes treated RNase free.
As above - you should use filter tips and RNAse free tubes, perhaps you should buy commercial RNAse free bottle of water, but for economy just use MiliQ. Clean your bench/pipettes with H2O2 3% solution if you don't have commercial RNAse eliminating detergents. It is also efficient.
Also, add to your reaction RNAse inhibitors and all will be fine.
DEPC-water is not for cleaning, it is for making solvents and using in reaction mixtures. Anyway, the advices are good. Use double-gloves, and change the upper ones sometimes.
If you want to clean your bench and make it RNase-free I recommend RNase away. This is a ready-to-use solution that can be used for surfaces, your gloves or pipettes or whatever. We purchase it from Carl Roth but I guess it is available from many other companies. You can purchase RNase-free tips and tubes from different companies as well but I know that these articles are very expensive. Normally it is enough to autoclave tubes and tips to make them RNase-free.
DEPC is just used to denaturate remaining proteins. After autoclaving this substance has no activity anymore.
All of the above plus everything that is heat stable -> 4 hours 160 - 200 °C all is sterile. I recommend it especially for all bottles etc for stock solutions.
I always rinse tips and all tubes in DEPC water overnight then autoclaving, that will be OK.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Biological Techniques