I want to extract and purify RNA from cell line using Qiagene RNeasy plus mini kit.
I have LC-MS spectra of extract where I have the PDF format of the spectra. I sent my extracts to a company who provided me with the pdf only (Not the original file of the software). The library...
02 March 2018 3,307 4 View
I have synthesized cDNA from 1ug of RNA using Quantitech Reverse Transcriptase RT Kit from Qiagen which is for Real Time PCR use. But in out lab we have Conventional RT-PCR. Can i use this cDNA...
07 August 2015 3,770 12 View
I am isolating compounds from plant. i have got 2 types of crystals,one is needle like and another cubic in shape in a vial. now from here how can i separate them? any technique??
03 April 2015 3,355 7 View
I have been synthesizing cDNA using QuantiTech Rev Transcrp (Qiagen) kit to see the expression of TFF1 gene. In the label of the kit box it says that this kit synthesize cDNA which is specific for...
11 December 2014 9,996 9 View
I have three Plasmids in glycerol stock. I labelled them and kept in -80 freezer for 6-7 months. Now when I wanted to take them out from the freezer I found the the labels disappeared. Now is...
07 August 2014 5,499 13 View
I am doing qPCR for TFF1/pS2 gene expression for the sample using reference compound Estradiol. As such, I am using gradient temperature for optimization. But I don't get any Band for my gene....
05 June 2014 1,178 27 View
I have done TFC. The procedure is as follow: # I dissolved 1 mg extract in 1 ml MeOH. # 100 ul of extract + 100 ul of 2% AlCl3 was mixed in 96 well and incubate for 15 min. # Absorbance was...
03 April 2014 2,146 2 View
I have primers from Integrated DNA Technologies in lyophilized form. The amount of Oligo is 25.5 nMoles = 0.16 mg. Now how should I dissolve it? Which solvent should I use? And how much of it?
02 March 2014 3,214 11 View
I have RNA , yield concentration 149 ng/ul in 50 ul stock. Now I want to synthesis cDNA using Quantitech Reverse Transcription kit (Qiagen). In the Protocol they suggest maximum 1ug of RNA to use...
01 February 2014 2,907 9 View
I am culturing MCF-7 cancer cell line in RPMI with phenol red and without phenol red for my experiment. 10% FBS is used. The passage is 42. For the last couple of passages I see that the cells are...
31 December 2013 9,038 11 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View