Do I need to make a special plasmid for bisulfite methylation test?

I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...

06 January 2021 7,218 2 View

How to perform cell transfection and Dual Glo assay with low-adhesive (HEK293) cells?

Hello everyone. I need to use Promega Dual Glo assay for HEK293 cells, but I am really afraid of wash out my cells from the 96-well plate, and at the same time, I am afraid to use coating because...

09 September 2020 4,817 7 View

How to reduce auto fluorescence in bone-marrow smears that would be used for FISH and IHC?

We are processing clinical samples of bone marrow smears, and have encountered autofluorescence that makes subsequent FISH and IHC virtually impossible. The problems are: 1. the samples are of...

18 December 2019 4,676 4 View

Antibody detects protein which was ablated in null mice (global knockout). Anyone had similar experience?

Here is a dilemma: there is a line of null mice (global knockout), which are null by genotyping and by mRNA analysis (qPCR). They were generated by replacement of an entire protein-coding region...

16 March 2019 9,722 6 View

Does high fidelity taq polymerase get less efficient after expiration date?

24 September 2016 5,468 4 View

Wrong or no fragment insert in directional TOPO cloning?

17 July 2016 10,020 10 View

Why in the analysis of cyanide, ninhydrin dissolved in sodium carbonate?

I was doing research related to the analysis of cyanide with ninhydrin, in some journals mentioned that preparation ninhydrin by dissolving it in sodium carbonate, but I do not understand why. Is...

12 July 2016 8,541 4 View

No title

15 June 2016 5,924 6 View

Why do I get a wrong length of PCR result but after sequencing it is actually a right target sequence?

Can somebody help me? I am doing a PCR of worm tissue. I used KAPA Taq Extra HotStart ReadyMix. I should be getting a PCR result of 158bp but I always get a 200 bp PCR result and after I sequence...

15 June 2016 7,313 6 View

What are the causes of PCR failure? i change the PCR master mix, optimized the annealing temperature and so on. but still no defined product band?

i want to amplified Channa striata COI gene using universal fish primer, out of 13 samples only two showed defined product bands after several optimization. I change the PCR mix, change the...

06 March 2016 854 6 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

Where can I obtain agricultural land use data for Wales, UK?

The Gower peninsula around Swansea is my target area

16 February 2021 7,473 3 View

How to differentiate the economic productivity in agriculture and in manufacturing sector?

Most of the references I found were on the economic productivity in agriculture but very limited application in manufacturing sector. I would appreciate if anyone could share the references on...

15 February 2021 8,342 7 View

What are the ways to test the knowledge level of agricultural labourer about their day to day work?

what kind of test can I do for testing the level of knowledge about their daily operation?

03 February 2021 7,583 10 View

What do you think about spatially based sustainable agriculture?

I am currently researching sustainable agriculture. I am interested in elaborating the LCA method and spatial mapping analysis. Most of the sustainable agricultural research only looks at 3...

17 January 2021 6,201 6 View

Which LCIA method is most suitable for assessing the impacts of nitrate leaching in groundwater (eutrophication )?

The eutrophication of groundwater by nitrate leaching is major environmental problem in Germany, especially in areas with intensive agriculture and high N-fertilizer inputs. In addition there are...

16 January 2021 5,182 7 View

Can you suggest literature studying the conditions information and evidence can influence manager decisions in social impact sectors?

I am working on improving the use of digital innovations at scale globally. Most of the innovations I work with provide relevant knowledge on improving the performance of projects, programs, and...

16 January 2021 1,120 5 View

What type of plants is better to work in gene regulation control project ?

I Interested in to work in gene network modeling thus I need to know what is future of gene network modeling in agriculture thanks

04 January 2021 6,527 3 View

No title

02 January 2021 7,172 4 View

Can you suggest Standard vocabulary for characterizing research and development initiatives (project,program,policy,networks) and their performance?

I am doing text mining on the performance on research for development interventions in low income countries. I need some standard dictionaries and typologies to map the content of the paper in a...

31 December 2020 5,329 4 View