Wrong PCR band size

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Wrong band size

Im having problem with a slightly wrong band size for one of my pcr products. I newly extracted the DNA therefore what could possibly be other reason besides template contamination?

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No title

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Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

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Prashant Bhaskar Kale

whether it is higher than expectation or lower?

if higher/multiples then, reduce MgCl2/Primer/template con.

It’s ok till the difference is of 10-15bp --->you carry on to next protocol...

The reason: this may be due to software handling (adding markers to gel picture….) and run parameters like gel/ladder used.

good day...

Hi BH Kong, i don’t think so!!!!, Please explain your case; What was the difference between observed and expected size?