Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Ferret PBMC Fc block?

Hi all, I'm going to run Flow Cytometery on ferret PBMCs but can't find an Fc block for ferret PBMCs. Does that even exist? Do I need ferret-specific Fc blocking antibodies or can I use:...

18 January 2021 4,504 5 View

Platelet Counting on Flow Cytometry?

Hi, Does anyone have a method for counting platelets on flow cytometry (cytometer=Cytoflex) without the need of using TRUcount beads or reference beads? Is it possible or do I need to purchase...

23 September 2020 3,509 1 View

What is the best bibliographic output style for preparing a CV?

I am looking for an output style in Endnote to list publications in one's CV. These would have to be be listed in reverese chronological order. Recommendations and opinions welcome.

23 March 2020 1,228 4 View

Anyone using stereo trail cameras (camera traps) linked to gather morphometric data from cryptic species?

Looking to connect with any research, papers or groups using remotely triggered stereo cameras to create 3 dimensional pictures of wildlife and gather data on cryptic species. Wanting to set up a...

01 February 2020 940 3 View

Finding EA(energy absorption)?

Hello every one, I am new to abaqus. How do I find energy indicators like, EA(energy absorption), Mean Crush Force (MCF) or Crush Force Efficiency (CFE) in a result section for a buckling process...

05 May 2019 5,193 3 View

What am I doing wrong - extracting a sapogenin for NMR?

I use hydrochloric acid to release a steroidal sapogenin from its saponin, then neutralise it with sodium bicarbonate. I am confident the hydrolysis works because the mixture no longer produces a...

03 June 2018 3,814 3 View

Unpuried PCR fine, Purified PCR smears?

Ran two Long Amp PCRs, one using un-purified template and the other a purified template. Un-purifed produces a PCR band and the purified doesn't. Negative control has no band or smears in it....

19 February 2018 2,456 4 View

How to detect chemical induced apoptosis?

18 September 2017 9,312 4 View

Unspecific binding in negative control ?

I have when doing methylation of DNA kit. when I do the negative control by not adding in the anti-5-methylcytosine antibody, then PCR, I am getting unspecific binding on my gel result, when there...

09 September 2017 9,668 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

02 March 2021 324 2 View

Does anyone have kinetic and kinematic dataset for gait classification?

I want to differentiate between normal and abnormal movement. For this reason, I am looking forward to this type of dataset.

01 March 2021 9,485 1 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View