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Dear colleagues, In my experience, some evaluations received in the peer review process of journals, often include criticisms based on the QUANTITY of examples you can claim you have in a...

06 November 2019 8,055 4 View

Where does the rationale on using AQL 2.5 and 4.0 standards come from?

I'm currently dealing with really large # of samples and want to set my AQL. So far, I've only seen these two as common standards for major and minor nonconformities, respectively. May I ask if...

19 August 2019 4,037 3 View

Which is the AAV2 ITR sequence?

Hello, I would like to know why some vectors contain this AAV2 ITR sequence, I want to remove that sequence together with others of my vector to make the stable transfection with lipofectamine...

15 August 2018 7,472 4 View

Are there any good tutorials or literature dealing with cluster analyses of categorical morphological traits at the species level?

I want to run a cluster analysis employing categorical morphological traits including all the described species of a genus of plants. My matrix includes both multi-state and unknown values for...

29 July 2018 3,772 6 View

[SOLVED] How can I solve the "Cannot read FoldX RepairPDB output" error in Yasara/FoldX?

I am trying to model point mutations in protein structures in Yasara using the FoldX plugin but I always get the same error: "Cannot read FoldX RepairPDB output". This is apparently a known error...

05 July 2016 7,716 2 View

I How can I obtain enough RNA from oral mucosa samples?

I'm attempting to extract RNA from oral mucosa cells. For this, I use a brush to obtain a sample and put it into RNAlater solution. Afterthat, I extract RNA with a special kit, but the results are...

06 April 2016 1,094 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View