Can we accumulate glycogen like polymer in sweet cassava?

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Dear colleagues, In my experience, some evaluations received in the peer review process of journals, often include criticisms based on the QUANTITY of examples you can claim you have in a...

06 November 2019 8,055 4 View

Where does the rationale on using AQL 2.5 and 4.0 standards come from?

I'm currently dealing with really large # of samples and want to set my AQL. So far, I've only seen these two as common standards for major and minor nonconformities, respectively. May I ask if...

19 August 2019 4,037 3 View

Which is the AAV2 ITR sequence?

Hello, I would like to know why some vectors contain this AAV2 ITR sequence, I want to remove that sequence together with others of my vector to make the stable transfection with lipofectamine...

15 August 2018 7,472 4 View

Are there any good tutorials or literature dealing with cluster analyses of categorical morphological traits at the species level?

I want to run a cluster analysis employing categorical morphological traits including all the described species of a genus of plants. My matrix includes both multi-state and unknown values for...

29 July 2018 3,772 6 View

[SOLVED] How can I solve the "Cannot read FoldX RepairPDB output" error in Yasara/FoldX?

I am trying to model point mutations in protein structures in Yasara using the FoldX plugin but I always get the same error: "Cannot read FoldX RepairPDB output". This is apparently a known error...

05 July 2016 7,716 2 View

I How can I obtain enough RNA from oral mucosa samples?

I'm attempting to extract RNA from oral mucosa cells. For this, I use a brush to obtain a sample and put it into RNAlater solution. Afterthat, I extract RNA with a special kit, but the results are...

06 April 2016 1,094 4 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Arbind K. Choudhary

Dear @Ancel Rei Salazar Acuña You can access the below mentioned link, and the attached pdf; hope, these would be useful to you.

https://www.researchgate.net/publication/313835831_Increasing_the_carbohydrate_storage_capacity_of_plants_by_engineering_a_glycogen-like_polymer_pool_in_the_cytosol

Yuan-Yeu Yau

🔴 Phyto-glycogen was reported. Sugary Maize (Su-1 maize) produces phyto-glycogen as it has a mutation in an isoamylase-type debranching enzyme [1]. So, if you mutate this enzyme artificially, you might be able to produce and accumulate (phyto)glycogen in plants (?). CRISPR-Cas technology is a good tool to produce such mutations.

[1] Article Extraction, isolation and characterisation of phytoglycogen ...