Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Langevin Dynamics in shear flow?

Hi, We are trying to simulate particles with Langevin dynamics in a shear flow by Lees-Edwards boundary conditions in MD simulations. It seems thermal forces and shear flow do not go hand in...

23 February 2021 1,761 1 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

Serum causes Hemagglutination in HI test. Virology Expert Please help how to resolve this issue while doing HI test for NDV and Avian Influenza?

I am doing HI Test for NDV and Avian Influenza Viruses but before going to start I first tested the serum whether any reactivity exists or not with 1% washed Chicken RBC. but I found that some...

11 January 2021 1,043 7 View

Do the tannic acid-treated sheep RBCs show agglutination with FAdVs (fowl adenoviruses)?

As we know that Adenoviridae indirectly agglutinates the RBCs of chicken, mice, rhesus monkey, and human blood type "O".However, do the tannic acid-treated sheep RBC show agglutination with FAdVs...

17 September 2020 6,630 3 View

How do you calculate your results in Bradford protein assay method?

I've used from Bio-Rad Protein Assay kit (Bradford technique), I measure my micro-plate absorbance in 595/450 nm ratio and I report the ratio blanked OD.Is it correct? And I usually see some...

09 October 2019 2,919 5 View

Can we consider hemagglutination assay for dengue virus as a quantitative method???

For a pure virus, HA can be considered as a way of representing their titre. even though it is cost effective and less labor intensive, it is less concerned. Kindly guide me in this matter.

06 July 2019 9,981 1 View

Can we consider hemagglutination assay for dengue virus as a quantitative method???

For a pure virus, HA can be considered as a way of representing their titre. even though it is cost effective and less labor intensive, it is less concerned. Kindly guide me in this matter.

04 July 2019 3,881 1 View

Replacement for erythrocytes (RBCs) for carrying out lectin - hemagglutination assays?

For an experiment I have to test for presence of lectins in a plant extract. I shall be carrying out the regular hemagglutination assay. However, I wish to avoid the use of erythrocytes. So I want...

03 April 2019 2,854 1 View

How to digest bacterial EPS at physiological temperature?

My bacterial biofilm tends to aggregate and agglutinate and I want to separate them at physiological temperature (25 - 30 C) in liquid. Any good suggestions of enzymes and preferably with...

28 January 2019 4,966 3 View

Is there hemagglutination inhibition activity in FBS/FCS/Bovine serum?

I am trying to detect anti-influenza antibodies in cell culture of human B cells using hemagglutination assay inhibition. I am wondering if FBS in my media may already have non-specific inhibitors...

09 October 2018 8,063 3 View

What is the role of lectins in the soil?

What is the role of lectins in the soil? In our studies we found in the soil proteins that have agglutinating activity. Their evaluation showed that they are lectins. Like soil enzymes, they may...

08 September 2018 5,257 4 View