The problem is that the extract is coming at different sides, not coming down smoothly. what should i do now. please reply as soon as possible should i continue, wash or do something else.
It is likely a column packing issue, or the column was allowed to run dry at some point. The best one can do is finish running the column and try again if your peak isn't pure enough.
May be your acetone has some drops of water. As Jack says you should finish running the column, concentrate the solvent, prepare another CC and run again. The is no other way.
Acetone is polar solvent and mixing it 1:1 cause rapid down of all soluble portion from column So, better start your column with 9: 1 (Pet ether: Acetone). You may also substitute your Acetone with Ethyl acetate.
I agree with Dr. Sethiya. Using Petroleum ether and ethyl acetate will be more useful. Even you can try Chloroform: Methanol combinations for the ethanolic extract.
Thank you all,,,,,,,,,,, the column was packed by wet method..... unfortunately the column was dried at some point.......... the solvent system was started from acetone:pet. ether (1:9) and was reached to 5:5 respectively...... however, luckily,, my column fraction has no spot at this point when i compared with eluent and extract and thus i washed.......... was it ok?
What I suggest is that you run a new column again. Pack the column using chloroform by wet packing method. Soak ur ethanolic extract solution (sample u want to run) and silica for at least half an hour. Your sample will get adsorbed in the silica and will not drain off suddenly. Allow to dry. Then pack the sample in the column with chloroform still on the top. Later remove excess chloroform from the top using a pipette. Then add the solvent system for elution. Your column will never break provided you use the solvents other than water. Good luck