With a new bacterial strain (after 16 s rRNA) expressing the desired protein, how can we design primers for that particular protein?

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Miro Svercel

Is the gene behind this protein known? The sequence of this gene? Is polymorphic? Than compare the different alleles and try to find places suitable for specific primer....etc etc...

Irsa Mateen

Protein is known but not characterized from this strain before!!!!

So then the pathway could be the same or similar as in other strains, used info published or known from other strains...genes, sequences and try to work on this..