Question about atomic charges on bio-molecule?

How to assign atomic charges on bio-molecules which were built by a Glycam Carbohydrate builder? The (.pdb) files generated by carbo-builder are without charges of the atom,? Any help would be...

03 December 2020 8,964 1 View

How to determine liquid film thickness (experimentally and theoretically), if the film is floating on another immiscible liquid?

I would like to determine the thickness of a liquid film floating (uniformly/non-uniformly) on another immiscible liquid. How to photograph the cross section of the film (in a non-invasive manner)...

03 November 2020 5,514 3 View

Is antigen retrieval step required for frozen spinal cord sections in IHC ?

I am doing co-immunostain using frozen spinal cord sections for IHC. Is it required to perform the antigen retrieval step prior to the blocking step ?

20 August 2020 4,144 0 View

After resubmitting the corrected version of my manuscript, why does it is showing as "Editor Assigned" for more than 2 months?

Based on learned reviewers given comments and suggestions I have made corrections of my manuscript and resubmit the updated version. In this stage why does it is showing as "Editor Assigned" for...

25 July 2020 5,162 3 View

Steered Molecular Dynamics Simulation using AMBER?

Hi all, I am performing steered molecular dynamics simulation of two polymer chains parallel to each other. Then, one chain has pulled using constant velocity and the trajectory I got is...

23 July 2020 7,805 2 View

Which one is floxed? What are the other methods to confirm it?

I have to collect mice embryos for my research and genotype them for KO gene. In PCR, the floxed band is always blur compare to control (control DNA from adult mice) (please see the attached...

02 June 2020 4,234 3 View

Why does the handling editor asked for major revision?

i. Although the suggested correction was very less raised by the 2 reviewers in their 2nd revision, why does the editor asked for major revision? ii. Why does the editor has given me clues to...

01 June 2020 711 12 View

Could anybody help regarding MD simulation using AMBER?

Hi All, I have build the model of cellulose polymer using xleap and hemicellulose polymer using glycam carbohydrate builder. Now, I need to place the two polymer chain parallel two each other....

20 May 2020 9,416 1 View

Is it possible to extract a particular feature from an image based on colour as a shape file using python?

I have a georeferenced image . Is it possible to extract a particular feature like drainage or roads from the geotiff based on its colour and save it as a shapefile using python?

16 March 2020 6,582 1 View

How to identify similar patterns in drainage shapefile using python?

I have a drainage/ river shapefile. If i identify a particular pattern/ feature in the drainage file then using it as input is it possible to identify similar features in the rest of the drainage...

29 January 2020 9,526 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

How can I obtain Reaction Forces in ABAQUS?

I am trying to simulate a simplified version of a beam resting on 10 points using Abaqus. The solutions I want to obtain are the Reaction Forces in each resting point or area when applying a...

21 February 2021 3,075 2 View

How to measure beam radius over some distance in Lumerical fdtd (Collimation evaluation)?

31 January 2021 6,354 2 View

How can I get oil properties in 300-30000K temperature range?

I need thermophysical and electrical oil properties to investigation of DC discharge in oil. Are multicomponent mixture models effective and precise in such calculations? If yes, what MC-modells...

04 January 2021 9,080 8 View

Hello everyone, which software is preferable for simulating the effect of shrinkage of metal powders for the Additive manufacturing process?

Last time I was asked about simulation software to analyze the effect of shrinkages of plastic powder materials. Currently, an additive manufacturing process is what I find suitable for my...

29 December 2020 4,615 9 View

When we regulate by tricolor light signals in VISSIM, should we make all the conflict zones inactive?

hi everyone, I have developed a simulation of a roundabout with a fixed time regulation, the lines of traffic lights in the roundabout are for vehicle storage. my question is: when we regulate by...

16 December 2020 1,923 3 View

How to find load / force acting on the top face in ansys workbench?

The image attached is the representation of my application. This is an assembly of three items (top plate, bottom plate and the 5 load cells). The bottom plate is fixed and a load of 40 Kg is...

15 December 2020 1,145 6 View

How to assign initial stress to a coating in Abaqus?

Hello! I am trying to define/assign initial stress to a coating I modeled on a substrate. I defined the coating using "skin" function. I have tried "predefine filed" in load section. But it only...

10 December 2020 2,921 8 View

How to extract patient specific 3D geometry from GE Vivid E95 3D/4D echo?

I am needing to obtain patient specific geometries of the left ventricle for use in CFD and 3D printing. Does anyone know how to do this using 3D/4D echo obtained from the GE Vivid E95? Does this...

29 September 2020 2,614 3 View