Question about atomic charges on bio-molecule?

How to assign atomic charges on bio-molecules which were built by a Glycam Carbohydrate builder? The (.pdb) files generated by carbo-builder are without charges of the atom,? Any help would be...

03 December 2020 8,964 1 View

How to determine liquid film thickness (experimentally and theoretically), if the film is floating on another immiscible liquid?

I would like to determine the thickness of a liquid film floating (uniformly/non-uniformly) on another immiscible liquid. How to photograph the cross section of the film (in a non-invasive manner)...

03 November 2020 5,514 3 View

Is antigen retrieval step required for frozen spinal cord sections in IHC ?

I am doing co-immunostain using frozen spinal cord sections for IHC. Is it required to perform the antigen retrieval step prior to the blocking step ?

20 August 2020 4,144 0 View

After resubmitting the corrected version of my manuscript, why does it is showing as "Editor Assigned" for more than 2 months?

Based on learned reviewers given comments and suggestions I have made corrections of my manuscript and resubmit the updated version. In this stage why does it is showing as "Editor Assigned" for...

25 July 2020 5,162 3 View

Steered Molecular Dynamics Simulation using AMBER?

Hi all, I am performing steered molecular dynamics simulation of two polymer chains parallel to each other. Then, one chain has pulled using constant velocity and the trajectory I got is...

23 July 2020 7,805 2 View

Which one is floxed? What are the other methods to confirm it?

I have to collect mice embryos for my research and genotype them for KO gene. In PCR, the floxed band is always blur compare to control (control DNA from adult mice) (please see the attached...

02 June 2020 4,234 3 View

Why does the handling editor asked for major revision?

i. Although the suggested correction was very less raised by the 2 reviewers in their 2nd revision, why does the editor asked for major revision? ii. Why does the editor has given me clues to...

01 June 2020 711 12 View

Could anybody help regarding MD simulation using AMBER?

Hi All, I have build the model of cellulose polymer using xleap and hemicellulose polymer using glycam carbohydrate builder. Now, I need to place the two polymer chain parallel two each other....

20 May 2020 9,416 1 View

Is it possible to extract a particular feature from an image based on colour as a shape file using python?

I have a georeferenced image . Is it possible to extract a particular feature like drainage or roads from the geotiff based on its colour and save it as a shapefile using python?

16 March 2020 6,582 1 View

How to identify similar patterns in drainage shapefile using python?

I have a drainage/ river shapefile. If i identify a particular pattern/ feature in the drainage file then using it as input is it possible to identify similar features in the rest of the drainage...

29 January 2020 9,526 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View