After heat shattering a DNA strand it into smaller strands (not denaturation), what kind of ends are produced? Are these able to be ligated?
Thanks!
The process of heating DNA at near boiling temperatures result in a loss of a purine base (termed depurination). The result is an abasic site, which is prone to spontaneous cleavage of the remaining phosphodiester bond.
My question is, will the ends of such a site be able to ligated?
As far as my understanding of the question goes, I think it will be blunt ended products otherwise one strand blunt ended and the ot.her sticky since the complementary pyramidine base is not affected by the heat. What is this used for? I also think you can try ligating them with blunt ended ligases, but the final product can be directionless if the heat shattering produces more than one strand
From PCR product that is right size band use as a template DNA for new PCR.
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