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Questions related from Damien P T
Or could someone kindly point me in the right direction with a link?Would be greatly appreciated..
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After heat shattering a DNA strand it into smaller strands (not denaturation), what kind of ends are produced? Are these able to be ligated?Thanks!
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I'm about to amplify 5 primers, and the melting temperature range between the 5 is 48°C to 68°C. Is there anything I can do apart from splitting the reactions up or changing primers? Thanks.
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