LC-MS could be used to identify the secondary metabolites present in the plant extracts.
Yes, I think so. Do you want to study the secondary metabolites content of the formulation? If yes, you can do it using LC-Qtof HR MS, By doing this, you can predict the exact MW of your metabolites,than using isotope pattern you can determine the molecular formula, after searching in the data base (eg. Metlin or others), and compared the fragmentation, I think you can predict possible secondarymetabolites the in extracts.
Yes, though it may be a complex mixture. I like ESI, run in both positive and negative mode, be aware of possible adducts (such as sodium) when checking the databases.
Here is an example for flash chromatography LC-MS:
http://www.isco.com/WebProductFiles/Applications/101/Poster_and_Paper_Reprints/New_Techniques_In_Extraction_Column_Screening.pdf
I think single herb itself multicomponent. Then if it is possible to single herb, then polyherbal may also follow this.
But try to find enough literature about chemicals present in individual herbs used in polyherbal formulation.
No.. Poly herb is a complex mixture, It is difficult to separate with HPLC. However, normal phase chromatography is suitable for this so that you will get partially purified compounds after that, perform reverse phase chromatography.
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