Based on some articles, it is recommended to store Vydac C4 column in organic solvent at least 50%. Will there be any effect to Vydac C4 if it is store in an organic solvent with concentration less than 50%?
It depends how long do you want to store this column. Generally it will be OK if you use solvent with at least 10 - 15% of organic component for short-term storage (e.g for weekend)