The Fe2+ and Fe3+ ions could very well quench the fluorescence in pyrene.The type of fluorescence (Static or dynamic) can ben established by a simple stern vollmer plot if you use PL spectroscopy for the study.
If you have modified the pyrene molecule
Fe3+ is rather well known and effective as a quencher for pyrene. The quenching process is generally believed to be dynamic and due to photoinduced electron transfer (the Fe3+ ion acts as a reversible oxidizing agent towards the excited state of the pyrene molecule). Examples of uses of this property have been reported in the literature (see links below).
As mentioned in the previous comment, a Stern-Volmer plot of the fluorescence emission intensity from monitoring a titration of pyrene (or its derivatives) with Fe ion will help you quantify the extent of quenching and the nature of the interaction (e.g. static vs. dynamic).
On the other hand, I would think that iron(II) might not be such an effective quencher, but I don't have literature to refer you to off the top of my head in that case.
Hope this helps!
http://pubs.acs.org/doi/full/10.1021/jo960191h
http://pubs.acs.org/doi/full/10.1021/ja980368k
http://pubs.acs.org/doi/full/10.1021/ol102543e
I think so. As commented, a Stern-Volmer plot will reveal you if the interaction is static or dynamic. This will depend on how the pyrenes are modified and what functional groups do they have sorrounding them.
I haven´t worked with pyrenees, buth structurally they are π-electron rich fluorescent aromatic compounds that probably will interact with electron deficient cations like Fe(III).
why Fe3+ is commonly quenches or interferes in the fluorescence interference studies ?
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