http://www.chemspider.com/Chemical-Structure.10216.html
I think it's unlikely, but I wonder why the compound in DMSO shows a different elution time than otherwise.
Dear Rohit,
This compound is unlikely to chemically reacts with DMSO . The elution time is a different story; it depends among other factors on the interaction of the compound with the solvent which hence affects the interaction with the stationary and mobile phases.
Rafik
It may be likely reacts with DMSO, because it is a universal solvent.
Could you please tell us more about the chromatography? Was it run on C18 or another column? What solvents were used? Was the sample dissolved in DMSO for injection, or was DMSO part of the mobile phase?
For C18, DMSO is a common solvent used to dissolve samples prior to injection. For non-polar compounds, elution seems to be unaffected. Very polar compounds may be affected. Run 458-36-B was a mixture of FD&C Yellow 5 (first eluting peak) and FD&C blue 1 that were dissolved in water and injected. 458-36-C are the same dyes run in the same fashion, except they were dissolved to the same concentration in DMSO. The yellow eluted earlier, the DMSO prevented the dye from interacting with the column. The runs used a flash C18 column. The experiment was run because I am interested in how dissolving samples affects their elution on C18.
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