My aim is to extract a compound (an oxopyridine) from plant material.I powder the material into chloroform and sonicate it for about an hour or two.
Is the compound susceptible to degradation? does sonication really aid the extraction?
Dear Rohit,
I don't think that sonication may corrupt components because there is no high temperatures. What we fear is the thermodegradationwhen we use high heat such as soxhlet.
hi rohit,
if you know your interest, do it right away. oxopyridine MP ^75 degree C. sonicate wont help much.
Soxhlet is better choice.
Ultrasonication techniques do help break the plant & microbial cells . As most of the plant extracts contain the aqueous systems, you may add a solvent like Ethanol along with the Chloroform in order to increase the miscibility of the Cell contents with the solvent system . Sonication will definitely help to bring the cell contents out into the medium but prior treatment of the plant tissues by maceration / cell disruption presses will be much better than the sonication alone .
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