15 March 2018 3 379 Report

Block chain technology is considered as next big thing for companies. Will this disrupt supply chain intermediaries?

More Prasad Kulkarni's questions See All
Issues with Malaria Coated Nitrocellulose Membrane Releasing Moisture?

Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...

16 July 2024 7,937 1 View

Exact mechanism of anti-cancer agents.?

What is the exact mechanism of synthetic agents showed the cell cycle arrest in G0/G1 and G2/M2 phase in flow cytometry analysis.

28 June 2024 5,950 2 View

Curcumin encapsulation in Bovine Serum albumin (BSA)?

Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...

23 June 2024 8,785 5 View

How to cite a paper?

I need to cite my paper that I have posted on research gate in other publication how do I do that?

21 June 2024 2,791 2 View

How can I implement GAFF-LCFF forcefield in GROMACS?

Dear experts, I am new to molecular dynamics and can run simulations on small Liquid crystal systems using GAFF in GROMCS. I wanted to use the GAFF-LCFF forcefield (Optimization of the GAFF Force...

11 June 2024 4,547 2 View

Albumin Nanoparticle Synthesis, troubleshooting?

Hey all I have tried synthesizing Albumin nanoparticles using desolvation technique using glutaraldehyde as a cross linker. After 24 hrs of stirring I could see that the protein has turned brown...

04 June 2024 1,501 0 View

How to publish a paper in the journal "CELL" ?

Is Cell the unit of living organism / life? Absolutely incorrect. Explanation: From fossils cell we have estimated , before how many years it was there, e.g 40millions years etc.. Was that cell...

28 May 2024 5,931 1 View

How to get 2 Octanol from Castor oil at a laboratory scale ?

Any latest method developed

24 May 2024 2,128 1 View

What are the different ways to apply the boundary conditions for a compact tension (CT) specimen in FEM modelling.?

Can we restrict the vertical and horizontal displacement in two directions for the lower semicircular in bottom hole of CT specimen and apply incremental displacement in the upper semicircular in...

09 May 2024 5,273 4 View

Who are the scholars who work at the intersection of religion studies and the built environment?

Who are the scholars who work at the intersection of religion studies and the built environment?

21 April 2024 1,195 3 View

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Handling Missing Data and Building a Predictive Model with Incomplete Information ?

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10 August 2024 4,005 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

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"A Markov-like Model for Patient Progression"?

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Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

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Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

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Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

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How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

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