Will acetic acid interfere the EDC/NHS coupling reaction between gelatin and Caffeic acid?

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Steingrimur Stefansson

Hi Abouzar, acetic acid will definitely compete in the EDC reaction, avoid it.

Use DMSO or DMF or buffers like Pi, HEPES, etc, that do not have a carboxyl or primary amino groups.

Abouzar Karimi

Hello and thank you.

You think It is possible to activate caffeic acid with EDC/NHS in another solvent, then add it to the main solution (with acetic acid) to react with NH2?