Why will NK-2 recombinantly expressed from Lactobacillus bulgaricus or Streptococcus thermophilus not kill the expressing organisms?

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Alaa Kareem Niamah

Hi,

The NK2 ( antimicrobial peptide) when production was linked with leader peptide series and There is a responsible gene.

Shireesh Apte

Thanks, but I am having trouble following your answer. I do have a signal peptide attached to the NK-2 to shuttle it out of the bacterium. However, my question is - what is to prevent the NK-2 from punching back into the bacterium via pore formation and ionic interaction?

Best,

Saba A. Hussien

Added file.