Nested PCR involves two sets of primers, used in two consecutive runs. This increases the sensitivity and specificity of parasite detection, and speed the diagnosis.

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 It allows detection and identification of low-intensity infections of protozoa

ncrease PCR sensitivity, avoid missed diagnosis in near species

Nested PCR involves two sets of primers, used in two consecutive runs. This increases the sensitivity and specificity of parasite detection, and speed the diagnosis.

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dear Mr. Noaman.. I hope this PDF answering  all your questions about PCR.

hello doctor 

Nested PCR is a modification of polymerase chain reaction intended to reduce non-specific binding in products due to the amplification of unexpected primer binding sites. it involves two sets of primers, used in two successive runs of polymerase chain reaction, the second set intended to amplify a secondary target within the first run product. This allows amplification for a low number of runs in the first round, limiting non-specific products. The second nested primer set should only amplify the intended product from the first round of amplification and not non-specific product. This allows running more total cycles while minimizing non-specific products. This is useful for very rare templates or PCR with high background.

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I think it is to increase PCR sensitivity .

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I think this method is highly sensitive

I agree with Dr . Aamer

Thanks for all who answer

Hi dr

I am so sory I am not especialist in this line.

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