Hi everyone,

I have analysed two genes localised next to each other in a phage. The analysis showed that the products of these two genes are interacting to each other to perform a specific function for the phage. I will refer to them as gene A and gene B based on their order in the genome of the phage.

What I found is that protein B was able to interact with protein A only when their coding genes (A and B) are cloned together (exactly as in the WT phage genome) into a plasmid under control of plasmid-encoded cadmium promoter.

However, protein B lost the ability to interact with protein A when protein B is over-expressed alone by the plasmid.

Indeed, when A and B both over-expressed by the vector, their ability to perform the function for the phage was very weak i.e. produced very few functional phage particles.

Any possible explanation for this behaviour please?

Cheers,

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