after I did a double digest process on the pET-28a plasmid using NdeI and BamHI-HF NEB restriction enzymes. However, the visualization results of the double digest have a semer in the double digest band. does this result indicate that the plasmid is not completely cut?

M (marker 1 kB)

D (double digest)

K (control)

More Awal Nur Rahmat's questions See All
Similar questions and discussions