Why the pressure go down to zero when i change the method?

03 March 2017 1 4K Report

The pressure is not stable

Probably because you have no flow of liquid, just air in the system. Please obtain some training and assistance in how to use the HPLC system from one of your teachers or the person "in charge" of the HPLC system. *Here is an article which may help you too.

"Diagnosing & Troubleshooting HPLC Pressure Fluctuation Problems (Unstable Baseline)"

http://hplctips.blogspot.com/2014/01/diagnosing-troubleshooting-hplc.html

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