What are the benefits exactly of synthetic intron (IVS) and internal ribosome entry site (IRES) on a plasmid?

Hello everybody! so I have a plasmid which has a synthetic intron (IVS) & an internal ribosome entry site (IRES), and I'm thinking to remove them as I'm cloning due to some matters related to...

26 January 2021 7,645 2 View

How we can calculated LOAEL and NOAEL for toxic pathological dose ?

how we can calculated LOAEL and NOAEL for toxic pathological dose

02 January 2021 1,175 2 View

What are the new methods of staining??

what are the new methods of staining??

25 December 2020 4,030 3 View

In XPS analysis of GO films. How I can use the sample work function method to know the information of XPS binding energy scale is referenced ?

Note that aligning the spectra to the C 1s peak of adventitious carbon is a highly risky procedure and was recently shown to lead to unphysical results. Therefore, I strongly suggest to avoid it....

18 November 2020 5,483 4 View

Dear Doctors/Professors, i would like to ask if a novel drug would be tested in animal model in mice which range of doses do i use first ?

Dear Doctors/Professors, i would like to ask if a novel drug would be tested in animal model in mice which range of doses do i use first ?

03 August 2020 3,811 3 View

I am working on COVID19 dataset??

I am working on COVID19 dataset, but, When I tried to convert the data.xlsx file to CSV file, I got an error. Also, i got an error when I upload the CSV file to WEKA. I tried to solve the problem...

05 May 2020 4,879 2 View

When I injected it into the LCMS all the chromatogram peaks are small lthough I have increased the concentration?

I have a metabolic extract from a plant, when I injected it into the LCMS all the chromatogram peaks are small, although I have increased the concentration several times but there is no change in...

12 March 2020 3,047 2 View

How can I do the Magnetic calculations using CASTEP code as ferromagnetic system , anti-ferromagnetic system ?

with a CASTEP

01 February 2020 1,612 4 View

How can i define the USRP N210 on pc under windows 10?

How can i define the USRP N210 on pc under windows 10 and i used gnu radio 3.8.. i set the configuration and i make the ping and recived reply successfuly but i cannot define the device and an...

21 January 2020 1,442 2 View

What is the number of measurement required in Circulant and Toeplitz sensing matrix in general in term of N an M in compressive sensing?

what is the number of measurement M (the number of measurement vector that result from multiplication of sparse vector N with sensing matrix (NxM)) required when using Circulant and Toeplitz...

16 January 2020 9,666 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View