Why the MTT, a tetrazolium salt is yellow? and why after its reducction by living cells is purple?... and finally, why the insoluble purple MTT formaz...

More Fernanda mo's questions See All

How to attach a videobeam into a microscope? Is it possible?

Good day everyone! I need to attach an image projector (videobeam type) to a compound optical microscope. Is that possible? Could you give me some equipment references (both microscope and projector)?

03 April 2019 4,921 2 View

When I work with medicinal plants, my major field of study is Natural Sources or Agricultural Development? choose one please

I work with natural compounds derived from plants and I search about their biological activity. Obviously plants are natural sources, but it doesn't mean that It couldn't generate an agricultural...

06 July 2014 8,739 3 View

How to report Blastocystis in human stool test? Perhaps, Blastocystis spp. cysts 0-1 per field?

02 March 2013 7,358 1 View

how you define a plant extract? what is its difference with an essential oil? Can you give some references?

08 September 2012 6,944 2 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal?

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal? Kindly advice

04 August 2024 7,367 3 View

Are authors sowing for scientific Journals to be reaping the benefits? We are charged for publication but we offer free peer review services, why?

As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....

03 August 2024 7,304 2 View

Is it true that science is leaving the era of mathematics and entering the era of matrix mechanics?

We assume this to be true. Science leaves the era of mathematics and enters the era of matrix mechanics and the turning point is the discovery of numerical statistical theory called Cairo...

31 July 2024 3,900 2 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

What are the important scholarly articles related to 'Use of Likert Scale in social science research' ?

The Likert scale is a commonly used measurement tool in social science research to assess people's attitudes, opinions, or perceptions. The social science students still have difficulty selecting...

25 July 2024 7,535 1 View

Is this a scam?

UK's Well-online Science Press sent an invitation to be a co-author and offers a payment of $30 per review.

22 July 2024 1,695 8 View

Is there a good macro photography kit for on-site metallurgical investigation?

I am looking to purchase a photography kit for onsite metallurgical investigations, such as capturing photos of worn areas, hot tears, cold tears etc. Could anyone suggest me a good...

22 July 2024 3,490 2 View

Hi, I am a research student at my university for the honours degree .how to find one?

Please if anyone could help me find a proper research question, because I am in a pickle of finding one in my data science field, I have no idea what to go with my research question. please...

20 July 2024 3,312 2 View

Christian Zuppinger

Recommended reading concerning your question:

http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/No.4_96/p14-19.pdf

Fernanda mo

Thanks for your reply. It helps me so much!. Have a good day. Fernanda Mo.

Bernhard Biersack

The MTT-derived formazan is not water-soluble because it is a neutral organic compound, thus it precipitates after MTT reduction and you need to add an organic solvent like DMSO to dissolve it again. The formazan has a quite different absorption spectrum compared with MTT, that's why the formazan is purple (it absorbs light of 500-600 nm). This effect bases on the different resonance (or mesomerism) of the formazan compared with the MTT salt.

Thaks Bernhard. Sorry by my late answer. But you really help me.