I am working on tissue culture of an epiphytic orchid. The asymbiotic seed germination was screened in different basal media (MS, 1/2 MS, K-C, G-B5, Mitra, etc.) and later sub-cultured in best basal medium in combination with different PGRs (Auxins, Cytokinins, Chitosans, etc.) However, the growth of in-vitro cultures are very slow. What could be the reason for slow growth?
The in vitro culture strategies have been developed that could work as useful tool to increase the biomass and yield of pharmaceutically important plants.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3550525/
Chitosan enhances growth and survival rate of in vitro-cultured plantlets of banana.
https://www.tandfonline.com/doi/abs/10.1080/15427528.2021.1889732?journalCode=wcim20
Dear Mihin Targu . See the following useful RG link: Article Germination and seedling establishment in orchids: A complex...
Kindly see also the following useful links: https://www.jstor.org/stable/2560340
https://www.scielo.br/j/hb/a/Txp8NmQrpvP3Fx3VQCHLscy/?format=pdf&lang=en
Also check please the following useful RG link: Article Effects of Different Growth Media on In Vitro Seedling Devel...
The following RG link is also very useful: Article In Vitro short to medium-term storage of two threatened and ...
Visit kindly the following useful RG link: Article The use of a new culture medium and organic supplement to im...
in fact, the develpment of orchids differ greatly between the genera (or even species). I would suggest to first look at similar species cultivation procedures
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