Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

Explain plasmid description?

Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....

14 January 2021 6,326 2 View

How big the Balb/c mice spleen after immunization?

I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...

04 January 2021 3,591 3 View

Capture of oleophilic solid polymers present in water solution ?

I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan

01 January 2021 5,798 3 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

Suggestion for normal ovary cell lines?

Dear all, I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks...

08 November 2020 9,100 3 View

What is Snapback Primer in realtime PCR?

Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.

01 November 2020 8,869 2 View

Rotation matrix - 3D dimension to 2D dimension?

22 October 2020 9,354 4 View

Am I introducing any potential loss of RNA integrity by combining an RNALater solution with a non-ionic detergent?

I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...

10 October 2020 1,830 3 View

How to calculate the acceleration/velocity of different molecules in an MD Simulation?

Hi, If I am performing an MD Simulation in which the two groups/molecules are moving in a direction on applying a force on the system, how would I calculate the velocities/acceleration of the two?...

08 February 2021 9,525 4 View

How to improve results of system identification procedure?

Hello, I'm performing system identification of the lateral closed-loop dynamics of a quadrotor. My model receives a setpoint and should return position and acceleration. I've proposed a second...

07 February 2021 9,791 2 View

How to get the natural frequencies and mode shapes using several acceleration data?

I am using FDD (code I used - https://uk.mathworks.com/matlabcentral/fileexchange/50988-frequency-domain-decomposition-fdd) to extract the natural frequencies and mode shapes using the...

03 January 2021 4,038 2 View

I am looking for a standard ?

Dear Researchers, I am a researcher and I am working on a Xenon accelerated aging of a polymer and I need the ASTM G 155 standard. I will be grateful if someone can share it with me. Best...

28 December 2020 549 2 View

Does anyone know if there is a faulty beaing signal generator available in MATLAB?

Hello everyone, I am trying to implement an algorithm to detect possible damage at a bearing. In order to test my algorithm, it would be helpful to generate faulty bearing acceleration signals?...

07 December 2020 1,483 3 View

How i can see the frictional and gravitational pressure drops?

Hi, i know the total pressure difference between inlet and outlet give me a total pressure drop in system, but we have 4 type of pressure drop in a system. gravitational, accelerational,...

28 October 2020 8,671 5 View

How to extract frequencies and mode shapes of a 3D bridge from installed multiple accelero-meter?

Dear all How can I extract the frequencies and mode-shapes of a 3D bridge from the acceleration record of multiple accelero-meters installed on the bridge? Thank you.

04 September 2020 119 6 View

Is ANOVA or Kruskal-Wallis applicable to test the group means, if each group has ca. 40 samples, but each sample is derived from 1024 sub-samples?

Dear Scholar Fellows, I would be interested in your opinion whether an ANOVA test (or Kruskal-Wallis-test) can be applicable in the following situation: a) 8 laps are driven on a test track,...

17 August 2020 887 3 View

Scanning Electron Microscope- How to correct crossover?

Hello, I have questions about XL30 SEM (with tungsten filament). Can anybody help me with how to correct crossover? You will see in the picture look of my crossover. I trying everything and can't...

24 May 2020 1,339 3 View

How to avoid procrastination while writing a research paper?

I was working for a project. The computational part is done. Now it's time to write a paper. But I am procrastinating much and actually I am not sure how to start or where to start.Which part...

18 May 2020 3,814 27 View