How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Tissue Dissociators for mouse spleens and lymph nodes?

18 January 2021 6,804 6 View

Servers for finding free energy and dissociation constant of protein-protein docked complex?

Hello everyone, I would like to get help about finding any server or application which can give me info about free energy and dissociation constant of protein-protein docking outputs. I have...

15 January 2021 587 3 View

How to calculating association and dissociation constants between two protein after MD simulation?

I am trying to calculate both association and dissociation constants between two proteins after MD simulation but my script isn't working. I need someone to kindly assist with script(s) or...

14 December 2020 3,110 1 View

Do I need collagenase to dissociate hepatocytes?

Hi all, after culturing hepatocytes in a monolayer on collagen in 24 well plates for 5 days, I attempted to trypsinize (0.25% trypsin) them to perform a cell count. However, none of my cells...

11 November 2020 2,478 2 View

Basic question - how to make a cell dissociation buffer?

Dear All, I'm looking to make a cell dissociation buffer from lyophilised collagen I (Gibco, 12648010). The protocols I've been looking at all specify concentrations of enzymes in terms of...

06 October 2020 506 3 View

Additional Questions about the dissociation constant, thank you?

Hello fellow scientists, I have some additional questions about the dissociation constant for a protein binding ligand. My main questions is if the protein concentration is much greater than the...

25 August 2020 2,814 8 View

How can one find the wavelength required for bond dissociation in a molecule ? What techniques are available ?

Particulary for the gas molecules trapped within 3D frameworks. What are significant parameters for calculations?

20 August 2020 6,212 3 View

Can you anyone tell the dissociation energy of LiF in terms of GPa ?

To calculate some elastic constants of glasses I require the above one...

14 July 2020 9,741 3 View

How to perform stable=opt during the geometry optimization process in Gaussian?

I am running a relaxed PES scan of a bond dissociation with Gaussian. Is there anyway to test the stability of the wavefunction (and optimize the wfn) at each step of the geometry optimization? Or...

08 July 2020 9,662 1 View