• Hematoxylin is a dark blue or violet stain that is basic/positive. It binds to basophilic substances (such DNA/RNA - which are acidic and negatively charged).
  • DNA/RNA in the nucleus, and RNA in ribosomes in the rough endoplasmic reticulum are both acidic because the phosphate backbones of nucleic acids are negatively charged. The negatively charged backbones form salts with basic dyes containing positive charges. Therefore, dyes like hematoxylin will bind to DNA and RNA and stain them violet.
• Eosin is a red or pink stain that is Acidic / Negative. It binds to acidophilic substances such as positively charged amino acid side chains (e.g. lysine, arginine).
  • Most proteins in the cytoplasm of some cells are basic because they are positively charged due to the arginine and lysine amino acid residues. These form salts with acid dyes containing negative charges, like eosin. Therefore, eosin binds to these amino acids/proteins and stains them pink. This includes cytoplasmic filaments in muscle cells, intracellular membranes, and extracellular fibers.

So, in optical microscopy we can observe :[1]

• Nuclei in blue/purple
• Basophils in purplish red
• Cytoplasm in red
• Muscles in dark red
• Erythrocytes in cherry red
• Collagen in pale pink
• Mitochondria in pale pink

• Hematoxylin is a dark blue or violet stain that is basic/positive. It binds to basophilic substances (such DNA/RNA - which are acidic and negatively charged).
  • DNA/RNA in the nucleus, and RNA in ribosomes in the rough endoplasmic reticulum are both acidic because the phosphate backbones of nucleic acids are negatively charged. The negatively charged backbones form salts with basic dyes containing positive charges. Therefore, dyes like hematoxylin will bind to DNA and RNA and stain them violet.
• Eosin is a red or pink stain that is Acidic / Negative. It binds to acidophilic substances such as positively charged amino acid side chains (e.g. lysine, arginine).
  • Most proteins in the cytoplasm of some cells are basic because they are positively charged due to the arginine and lysine amino acid residues. These form salts with acid dyes containing negative charges, like eosin. Therefore, eosin binds to these amino acids/proteins and stains them pink. This includes cytoplasmic filaments in muscle cells, intracellular membranes, and extracellular fibers.

So, in optical microscopy we can observe :[1]

• Nuclei in blue/purple
• Basophils in purplish red
• Cytoplasm in red
• Muscles in dark red
• Erythrocytes in cherry red
• Collagen in pale pink
• Mitochondria in pale pink

Hi

I full agree with Dr. Atiaf

I think this research help me with my work

thank you very much

Hi

I full agree with Dr. Atiaf

regards

I agree with Dr. Atiaf

iagree with dr Furqan

many thanks

Hematoxylin is a dark blue or violet stain that is basic/positive. It binds to basophilic substances (such DNA/RNA - which are acidic and negatively charged Eosin is a red or pink stain that is Acidic / Negative. It binds to acidophilic substances such as positively charged amino acid side chains ).

good question

Hi

Agree with Dr. Atiaf

many thanks

Dear Dr. Hala

I agree with Dr. Atiaf

regards

many thanks