Why some probiotics strains intially tolerating bile starts decling in tolerance after 24 hours?

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Tariful Islam

Selecting justifiable bile concentration could be a player in your case. You may try 0.3% bile salt for a better results.

https://www.researchgate.net/publication/262690130_Analysis_of_antimicrobial_activity_of_Lactobacillus_paracasei_ssp_paracasei-1_isolated_from_regional_yogurt

Article Analysis of antimicrobial activity of Lactobacillus paracase...

Ashwini Kumar Dubey

Bile tolerance in probiotic bacteria is strain specific. A probiotic strain with bile salt hydrolase activity is preferable. Ensure that the cells what you have used are in the late log phase or in the log phase.

You can run several replicates with the control.

Regards,

A K DUBEY