How could I get the P-value from the mean, SEM, and n of different groups in the t-test?

How could I get the P-value from the mean, SEM, and n of different groups in the t-test without the original data? For detail, I would like to know the P-value between BMP-2: 50ng/ml group and the...

06 February 2021 6,539 4 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View

What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...

26 December 2020 9,921 2 View

Can Apparent Activation Energy determine Conversion Rate in a catalysis reaction?

Recently, I got a concept that is contrary to my understanding from two papers. It can be described as follows, in brief: There are two catalysts for the same reaction, on the catalyst with higher...

09 December 2020 2,406 28 View

What is the standard preparation procedures of Powder X-Ray Diffraction (PXRD) sample?

Recently I started to use XRD to study the structure of some molecular sieves. My powder sample was prepared by grinding in a mortar, drying in an oven, and loading on the sample holder. However,...

03 December 2020 7,504 8 View

Could anyone professional help me to solve this problem?

I have installed the software BRUKER Flexanalysis 3.4 on my computer successfully. But when I tried to import the MS data produced by BRUKER MALDI-TOF-MS, I met with an error described in the...

10 November 2020 8,205 1 View

How to prove the hypermethylation of a certain gene inhibits the gene expression?

Hi, As described, recently we found a gene was silenced in various tumor, and its promoter was alwasy hypermethlated. When using certain treatment to induce the gene expression, we found its...

04 November 2020 7,054 3 View

Could anyone recommend a kit to isolate exosome from cell culture supernatant?

Hello, I'm working with immortalized HUVECs low and I'm trying to study their exosomal RNA contents. Could anyone recommend an effective kit to isolate exosomes from cell culture supernatant?...

31 October 2020 4,252 5 View

Is there a technique to implant a cannula for repeated CSF sampling?

We need to collect CSF samples repeatedly from the 4th or lateral ventricle of rats. Does anyone know of a protocol for implanting a cannula to be able to do this? Or even a protocol for this...

26 October 2020 5,804 3 View

Any ideas on how to prevent the mouse cornea cryosections from dehydration?

I am analyzing the tissue damage to the mouse cornea tissue after incubated a kind of medium. The protocol is 1) mouse cornea was incubated in the medium for one day 2) embedding the cornea with...

21 October 2020 7,568 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View