Why semiconductor optical amplifiers impose a chirp on the pulse during amplification.?

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Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

Can't spin-orbit coupling(SOC) be included in optical properties calculation in CASTEP?

I am currently using CASTEP version 2020. When I want to run optical properties calculations with spin-orbit coupling(SOC), I encounter the following error in a pop-up window before the job gets...

02 August 2024 2,890 0 View

When Using SYBR Green for Flourescent Detection of LAMP Products what causes an Overshoot in Flourescence Reading?

The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65...

14 July 2024 1,483 0 View

I am carrying out an experiment on Trichoderma. How can I measure OD of the fungal growth after 7 days?

I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....

11 July 2024 6,251 1 View

Can some indicate a technique - or approach - to measure the thickness of a 20µm film and thickness with water uptake?

Hi, I need to measure the real-time (or temporal intervals) thickness variation of a transparent material from dry to wet/humid conditions, however, I cannot use the following techniques: -...

08 July 2024 4,799 4 View

How to solve Bubble Error in fiber splicing?

I'm having a bubbling error while splicing 100/350 um optical fiber (core/cladding) on the Fujikura FSM100P+. I have tried some ways such as changing Prefuse power and Prefuse time but to no...

03 July 2024 7,463 6 View

When using the relative quantification method in the 7500 PCR system, is a reference sample essential?

Hello. In our laboratory, we are currently conducting Loop-Mediated Isothermal Amplification (LAMP) to detect insert DNA in plasmid vectors. When using the LAMP method, we typically use 8 tubes,...

24 June 2024 645 1 View

What could be the reason for PCR amplification failure using Whole genome amplification products??

I have designed primers for HBB Gene and the template is Whole Genome amplification(WGA) products. The primers work in some samples and in some they don't. I measured the OD of both the samples....

23 June 2024 3,362 2 View

What is the relation between "Depth of Field" and "Resolution" in microscopy ?

Please explain with reference to the attached figure. Also, What does "infinite resolution" of a microscope (optical or electron) imply? Similarly what do we mean by "zero resolution"? Does having...

04 June 2024 711 4 View

Title: Single Band in Positive Control LAMP Assay - How to Fix?

I am conducting a LAMP assay, and while the negative control shows no bands as expected, my positive control samples consistently display only a single band instead of the anticipated ladder-like...

18 May 2024 4,853 3 View

How to calculate the angular velocity of a rotating cell from a microbe video?

I have a video of Brownian motion of microbes under a microscope. From this video, I need to calculate the angular velocity of a particular prominent cell. From my understanding, what I know is...

18 May 2024 4,289 3 View