03 February 2022 2 5K Report

Hello. I have a question while western blot.

I think I have problem with my protein extraction.

First 4 lane on this picture is mine and last lane is other lab member's.

Only mine looks smear.

I used same protocol and buffers with others.

I added RIPA buffer on ice and wait for 20min on ice and scrapped it.

And then I did centrifuge for 14000rpm 20min.

Can you suggest what's the problem in my protein?

More Jisoo Shin's questions See All
Similar questions and discussions