Hello. I have a question while western blot.
I think I have problem with my protein extraction.
First 4 lane on this picture is mine and last lane is other lab member's.
Only mine looks smear.
I used same protocol and buffers with others.
I added RIPA buffer on ice and wait for 20min on ice and scrapped it.
And then I did centrifuge for 14000rpm 20min.
Can you suggest what's the problem in my protein?