Typical concentrations of glucose transporters in mouse brain?

I have measured levels of GLUT1, GLUT3, and GLUT4 in the mouse hippocampus and cortex using ELISA kits. I'm trying to find typical concentrations (in ng/mg total protein) of these proteins in the...

21 December 2020 4,647 2 View

How Shall I perform Principle Component Analysis in GROMACS?

I have to perform PCA as a part of my results and analysis of protein-ligand complex docking using GROMACS 2018.8. How shall I perform PCA and interpret the results for the same? Any insights...

13 December 2020 3,121 2 View

How shall I prepare a protein PDB file in a particular pH for docking and simulation?

I wanted to prepare a protein at an alkaline pH for molecular docking as that is the pH I want to perform my in vitro assays in? I prepared my protein at the required pH through H++ server;...

13 December 2020 2,272 3 View

I need help in peptide oxidation during deprotection group removal?

Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...

03 December 2020 3,225 2 View

How to identify GC peaks for FAME mix which do not match reference peaks?

Hello, I am analyzing 37 component FAME mix using GC. I have got 37 peaks and 25 of them show expected order and height. However, others are not in the similar order with the reference for same...

22 November 2020 7,033 4 View

What could be the many interpretations of the Bass Model?

The Bass model has drawn a lot of research over the years and subsequent research has interpreted the model in numerous ways like Lekhvall and Kahlani. I would love to know any other ways the...

03 November 2020 6,434 1 View

Which of the five PDB files (DRGFIN.PDB, DRGMMX.PDB, DRGNOH.PDB, DRGPOH.PDB & DRGAPH.PDB) as an output from PRODRG Server do I need to use?

I have some protein-ligand complex to simulate on GROMACS version:unchecked. For preparation of my ligands, I submitted the extracted Ligand.pdb file and submitted it in the Prodrg server, Energy...

01 September 2020 9,154 5 View

Online questionnaire on Impact of Covid-19 on Human Resources?

Dear Friend/Sir/Madam We are conducting an online survey as mentioned in the subject, please fill it and submit. Please also forward to your esteemed group. The questionnaire aims at seeking...

12 June 2020 1,505 4 View

I want to perform immunohistochemistry experiments in order to find Cancer stem cells using Hoechst 33342 dye. Can anyone give me a protocol?

I would like to visualize cancer stem cells in breast cancer tissues, isolated from patients, using Hoechst 33342 stain. If anyone can provide a link to the protocol, it would be very helpful.

12 March 2020 4,268 2 View

How to convert Rice gene IDs to NCBI gene IDs ?

For scientists working in rice, is there any converter/annotation file to convert RAP DB/ MSU IDs to NCBI gene IDs ?

03 April 2019 3,324 4 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

In which case meta-heuristics algorithms cannot minimize the network error?

Hello, I am working on hybrid neural network. I have combined ANN with two different evolutionnary algorithms to build a prediction model of 6 inputs and one output with one hidden layer of 7...

18 January 2021 7,144 3 View

What is the most suitable experimental design?

I am planning to do a study with the help of 45 farmers who are going to produce hybrid chilly seed for the first time, and I want to measure in what way semi-automated greenhouse will affect on...

14 January 2021 2,799 4 View

Hi, what is the problem , I am unable to upload my Research article. It IS asking only authors can upload while I am author?

12 January 2021 3,050 5 View

What are the dominant factors contributing to the success of electric vehicles in the marketplace?

Early innovation by Toyota via the Prius established an early adopter crowd for electronic vehicles. Since, competition in many offerings: GM (Volt, Bolt), Nissan (Leaf), and the rise of Tesla set...

02 January 2021 1,787 13 View

How to Interpret Photoluminescence Spectrum of Mixed Pb-Sn FA0.7MA0.3Pb0.7Sn0.3I3 Perovskites?

Dear Researchers, I hope you all will be in good health. I am writing this to request for your guidance. I am working on the fabrication of low bandgap Pb-Sn mixed perovskite with exact...

23 December 2020 1,147 1 View

Transgenic or Mutagenic organisms, which is more stable genetically?

Hello, Which of these genetically altered organisms is more suitable for Scientific research? Is it the Transgenic hybrids or the Mutagenic organisms? Which is more genetically stable to be used...

16 December 2020 2,049 3 View

What are best conditions for in vitro hybridization?

Hello, I would like to hybridize a probe and an anti-probe (ssDNA oligonucleotides) in a reaction vessel. What would be the best buffer to use? and the temperature of annealing? Thank you

06 December 2020 4,121 0 View

Whats wrong with my cells ? contamination ?

Hello everybody, i hope somebody can help me with my cells. Since 2 weeks they started to change morphologically, as you can see in the two pictures with the smaller magnification (they started...

18 November 2020 1,183 3 View

How to form a quasi-ominidirectional beam with antenna arrays?

For beam alignment (802.11.ad) in a mmWave MIMO system, the transmitter starts with a quasi-ominidirectional beam. My question is how to design the hybrid precoder for a quasi-ominidirectional...

11 November 2020 7,516 3 View