My samples and molecular weight standard were dragged in the western blotting electrophoresis running. In the comassie staining I can not see the proteins and you can see that the molecular weight standard was also not separated.
It is not getting resolved primarily. The concentration seems to be quite low. Seems like the protein may have just flowed right through the gel and you may have lost the proteins. Maybe try increasing the concentration of your gel to help resolve your protein.
Dear Renata,
As your query is not provided in details. So, there are some possibilities for that.
1. Please check the composition of you gel including pH;
2. Check the composition of sample loading buffer;
3. As mentioned by Sam Mathew that please make sure there is enough protein.
Given the fact that even the standard was also not separated. I suggest you to check your gel first.
Good luck!
Jianing
Is the line seen in the top image the dye front or a wire? If it si the dye front, then electrophoresis seems to work ok, including focussing in the stacking gel. If it's just a wire, then little can be said from the photos.
I'd try to debug the electrophoresis first with a cheap sample like serum, perhaps you can get help from an experienced colleague. I have published a very detailed protocol for SDS-PAGE in doi:10.1007/978-1-59745-542-8\_14, if that helps.
- Badges
- Science topic
- Similar topics
- Biological Science
- Kinesiology
- Running