Why my insert's exon is not being included in the cDNA from my pSPL3 construct?

05 May 2016 1 1K Report

I am trying to perform a minigen assay using the pSPL3 vector. The results showed no inclusion of my insert's described exon, but the same cDNA is obtained from the empty vector than from the vector containing the WT exon sequence. Has anyone had this problem before? Which do you think it can be the trouble? Thanks.

Ajay Saini

Hi Barbara,

Can you elaborate the set of experiments a little bit that you carried out

bye

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