How to purify gallic acid to get white crystals?

I have isolated gallic acid from triphala churna but it is light brown in colour.

11 January 2021 8,737 1 View

How to calculate cell doubling time from confluecy of cells?

If i am Knowing the confluency of cell culture, is it possible to calculate the doubling time of cells.

23 December 2019 7,456 4 View

How to avoid the interference signals arising because of the wires in Digital oscilloscope?

I am trying to make pressure/force sensors and measuring the corresponding signals on digital oscilloscope (DSO). But most of the time observing the interfering signals because of the wires/clips...

27 November 2019 3,169 5 View

Can someone explain the formulae highlighted (yellow) on page 5 in attached file?

I am working on symbolic data analysis. Can someone explain the formulae on page 5 (highlighted in yellow ) in attached file?

31 May 2019 6,700 3 View

How to implement an algorithm in Cloud Sim Too?

I have a doubt regarding CloudSim. What ever examples are given. All give same fixed results. Even I run N times. What changes I should do so that it give different results.

12 June 2018 3,346 3 View

How to interpret spread-ability and extrudability data obtained by stable micro system?

I have prepared a gel formulation and evaluated it by using stable micro system please help me for interpreting the data for spread-ability and exrudability

28 December 2017 6,094 2 View

Can we perform NS1 antigen detection from cell culture fluid?

I have C6/36 cell lines infected with serum sample. Can i do NS1 antigen detection in supernatant culture fluid? Does it requires purification step?

19 November 2017 7,362 1 View

Can any one suggest me how to analyse unbalanced two way ANOVA in SPSS and which post hoc test is suitable for this kind of analysis?

If any literature regarding unbalanced two way ANOVA is available please share it to me. Thank you

05 November 2017 9,989 6 View

Which is the most reliable method for checking spreadability of topical gel formulation?

03 August 2017 3,279 1 View

How much maximum time GA usually take to complete the iterations?

23 April 2016 4,151 17 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View