I extracted these cells from donated adipose tissue as usual protocol: 1mg/ml collagenase for 1h and cell strainer 100 and 70. In culture with DMEM high glucose + 10% FBS. They are in 2 passage in the picture. At day 1 and 2 they present a fibroblast like morphology like seen in the picture but as the days go by (4-5 days a culture) they expand with what looks like cytoplasm prolongations. And they are not dead because when I add trypsin to expand them, changing the dish they go back to the fibroblast like morphology and starts again with the weird morphology.
Hi Jiulia, from your pics it looks like you have a heterogeneous population with senescent cells. But they do have fibroblast-like morphology! I would add 10-20ng/ml of FGF-2 to help... Anyway you should obtain a homogeneous population after 2-3 passages in culture. Which passage are you cells in the figures?
Best,
Yuri
I agree with Yuri. There are some fibroblastsort in the two fields you attached. However there are some other cells too.
They are 2 passage. At first they all look like fibroblasts, just like the population on the second picture but then in 4 days they all start to transform like the first picture, and they are not dead, but I don't why they expand in this way
they are infected by mycoplasm? Use a medium containing an mycoplasm antibiotic.
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