What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View

Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View

How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

Why can I not enter the title of the journal?

When I upload articles the details page does not allow me to enter the names of lesser known journals. It says "type to search" but because the journal is not on the list, I am left with a blank...

26 January 2021 2,988 3 View

SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

24 January 2021 3,791 4 View

What is the ACTUAL COST OF A PUBLICATION??

Hi. It's 2021 January. Recently, I got to know that Elsevier has converted around 160 journals into some "Transformative Journals" and follow the recommendations under "Plan S"...

19 January 2021 9,985 2 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

How to standardize and set parameters in the Puller sutter P-97 to get accepted pipette shape and resistance for whole cell recordings on DRG?

We are working on a P-97 puller and we are using it to do whole cell recording in DRG. We have 2 types of pipettes (borosilicate glass ID: 1.1 OD: 1.5 and ID: 0.86 OD: 1.5 ) as it showed in the...

04 January 2021 2,112 6 View

QPCR considerations in analyzing samples from the field?

I am attempting to plan a qPCR experiment in which I evaluate the expression of multiple genes in various brain regions of fetuses, as a function of location in utero (intrauterine position,...

31 October 2020 5,788 1 View

Detection of Rat X and Y chromosomes by PCR?

Hi, I was wondering if anyone has the protocol for rat X and Y chromosome detection by PCR particularly Digital PCR. I want to quantify the actual amount of X and Y chromosome from the same...

14 October 2019 9,257 3 View

Detection of Rat X and Y chromosomes by PCR?

Hi, I was wondering if anyone has the protocol for rat X and Y chromosome detection by PCR particularly Digital PCR. I want to quantify the actual amount of X and Y chromosome from the same...

09 October 2019 9,111 4 View

Difference between Digital PCR and Droplet Digital PCR ? Pros and Cons ?

I'm going to perform gene expression analysis by digital system .. which platform should I choose ?

09 October 2018 7,971 0 View

How to interpret digital PCR scatter plot?

Hello everyone, I am unfortunately getting sub-quality results from my dPCR runs where the clustering (i.e. fluorescent amplitude) of the FAM and VIC assays are not separated. Essentially, I am...

23 March 2018 2,654 3 View

How to interpret digital PCR scatter plot?

Hello everyone, I am unfortunately getting sub-quality results from my dPCR runs where the clustering (i.e. fluorescent amplitude) of the FAM and VIC assays are not separated. Essentially, I am...

02 March 2018 1,230 4 View

Real-time PCR instruments ?

We are going to purchase qPCR instrument to our new genomic platform; Which the better choice regarding specifications, medium throughput applications and price between this instruments : qPCR...

20 February 2018 2,867 3 View

Real-time PCR instruments ?

We are going to purchase qPCR instrument to our new genomic platform; Which the better choice regarding specifications, medium throughput applications and price between this instruments : qPCR...

01 February 2018 3,532 5 View

Does anyone have experience with rare mutation detection by using HEX assays (not VIC) on QuantStudio3D dPCR system?

We are trying to adapt FAM/VIC-based custom assays to FAM/HEX (or YAK) for rare mutation detection on our QuantStudio3D dPCR system. Does anyone have already tested this possibility on this dPCR?

08 September 2017 5,915 0 View

How to calculate DNA copy number from digital PCR?

In the denominator, where is ln(1-1/C) from?

01 February 2017 7,996 1 View