Hi guys,
I'm a little bit in trouble with LTP on CA1. I performed some experiments inducing LTP after HFS (1 for 100 stimuli at 250Hz). This protocol is able to induce a strong potentiation of the synapse. My problem is that the potentiation after the induction is not stable and the response continues to increase without reaching a plateau. What do you think the problem is? I recorded extracellularly fEPSPs from hippocampal brain slices (400uM thickness; flow rate of about 2 mL/minute).
Thanks in advance for your advices.
Luca