Leucine uptake assay is widely used for bacterial production. But I have three concerns (1) Why Leucine (not other amino acids) is used and (2) In case of environmental samples, Leucine may be present in water and in that case, how to say that Hot leucine (radioactive Leucine) was only incorporated into the bacterial proteins or how to negate the uptake of environmental leucine from incorporation? (3) Does all proteins incorporate Leucine in case of bacteria?
The molar fraction of leucine is similar in various prokaryotic proteins. You may be interested in the following early two papers.
https://aem.asm.org/content/aem/49/3/599.full.pdf [PDF freely downloadable]
https://doi.org/10.1111/j.1399-3011.1973.tb02325.x
Nobuyuki Hamada do you know what does it mean dpms units in a graph where leucine and thymidine was incorporated to a bacteria?
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