We recently constructed a plasmid vector with a high-copy number ori, kanamycin resistance, and eGFP under control of the lac promoter but lacking the lac operator. However, the cells are not exhibiting any fluorescence. We purified the plasmid and had it sequenced via Sanger Sequencing, and the results of sequencing show that the plasmid has the correct, expected sequence. What could prevent expression and/or subsequent fluorescence of eGFP?

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