I am working phosphate soluble bacteria. I am interested in estimation of Phosphate soluble by spectrophotometer. But I am getting problem on preparing Pikovskaya’s Broth as it is getting precipitated. I am using double distilled water with TDS 3.
Hopefully, NBRIY and NBRIP broth medium will resolve your issue.
Dear ALi,
It is always turbid as TCP is not soluble. Nothing unusual in it. Just swirl the medium before pouring plates.
Dear Ali, Phosphate solubilization means dissolve of insoluble phosphorus. Pikovskaya’s broth contains insoluble TCP after inoculation of bacteria at desired time interval spin down the broth for 10000 rpm for 5-10 min, and carefully collect supernatant and estimating soluble P from supernatant.
Dear
This is because of presence of TCP in the PKV medium and it is better to use NBRIP (National botanical research institute for phosphate solubilization) medium for estimation of Phosphate solubilizing bacteria. Preparation of the medium/inoculation of sample/incubation at 370C/3days/pick up 1ml sample and centrifuge it 5000rpm for 7min./addition of PNP indicator and sulfomolbdate solution and sulfuric acid and stanus chloride/ then take od at 570nm.
Dear Ali
As said by many researchers in above answers, Pikovskaya (PVK) is turbid because of presence of TCP. You can estimate or quantify Solible phosphate from cultural supernatant after centrifugation of the culture.
Tris rock phosphate agar (TRP) or Buffered Rock Phosphate agar is another medium which is best for the final confirmation of a Strong MPS phenotype. Several bacteria show MPS phenotype on unbuffered media like PVK or NBRIP but fail to solubilize Rock Phosphate (soil phosphate), and hence may also fail field condition.
I recommend to test MPS phenotype of your isolates on TRP.
- Badges
- Science method
- Similar topics
- Biological Science
- Ecology